Gram staining is a differential stain that helps in distinguishing between types of bacteria and it is the most useful staining procedure used today (seeley and others 1991) in this kind of staining procedure, a basic dye, a mordant, a decolorizing agent and a counterstain is needed. Most differential stains have a challenge step that follows staining with a primary dye in the gram stain the challenge step is a rinse with either ethanol or acetone (either may be used) this step dehydrates and tightens the cell wall of gram positives (mainly peptidoglycan) such that the rinse does not enter the cell. Differential staining relies on the bacterial cell wall structure in order to differentiate the gram negative from the gram positive the latter has thick peptidoglycan stratum in the wall that has the ability to hold on to crystal violet stains following acetone or ethanol exposure or both at the same time. A differential stain involves the use of two or more stains depending on the components of the bacterial cell wall or outer layers, the bacteria will either retain the primary stain or have the primary stain removed in a decolorizing step and then retain the secondary stain. Free essays on differential staining for students use our papers to help you with yours 1 - 30.
Purpose, procedure and preparation gram stain is probably one of the most commonly used staining procedures used in the field of microbiology it is one of the differential stains that are used to characterize bacteria in one of two groups: either gram positive bacteria or gram negative bacteria. In differential staining the one type of stain that is very useful is the gram stain gram stains are done in order to distinguish bacteria based on the composition of cell wall they have they are two types of gram stains the gram-positive and gram-negative. The gram stain is a differential stain that allows you to classify bacteria as either gram positive or gram negative this gram stain technique was discovered by hans christina gram in 1884 we will write a custom essay sample on gram staining specifically for you for only $1638 $139/page order now the purpose of this lab was to.
It is one of the most important and widely used differential staining techniques in microbiology this technique was introduced in 1884 by danish physician christian gram gram staining procedure is illustrated in fig 52. It is a differential staining method of differentiating bacterial species into two large groups (gram-positive and gram-negative) based on the chemical and physical properties of their cell walls. A differential staining technique is a procedure that allows the observer to visually distinguish between different types of bacterial cells based on the idea that not all cell types stain equally. The differential staining method enabled visualization of the thicker cell walled, gram-positive bacteria staphylococcus and lactobacillus, as well as the purple staining of the yeast s cerevisiae the color of s cerevisiae is noteworthy, as it is a fungus. Differential staining techniques are used to help identify microorganisms based on the morphology and its ability to accept the stains used during the staining process the cells retaining the initial stain are positive, and the cells retaining the counter or secondary stain are considered to be negative.
Differential staining essay differential staining purpose: the purpose of this experiment was to become familiar with subtypes of culture media and the uses for each, learn and employ the streak and pour dish techniques, and generate a pure culture of a specific organism. Published: wed, 30 may 2018 introduction the purpose of this lab was to identify unknown bacteria cultures using various differential tests the identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based. Compilation of microbiology staining q&a essay sample identification and classification of bacteria is important to make easier manipulation on the bacteria for various purposes such as for medical, research, developmental, and biotechnicalfieldsthe cell wall is the basis for classification of bacteria according to the gram stain. Differential staining a describe the differences between gram-positive and gram-negative cell walls the cell walls in the gram-positive bacteria are thick, with interconnecting layers of peptidoglycan peptidoglycan is a polymer, mesh like sugar and amino acid layer forming the cell wall of a bacteria. A differential stain that uses crystal violet, iodine, alcohol, and safranin to differentiate bacteria positive bacteria stain dark purple negative ones stain pink or red differential stain.
Gram staining is a differential or specific method of staining gram staining is a way to tell the difference between gram positive and gram negative bacteria the cell wall of the gram positive bacteria is made up of several “layers of peptidoglygan,” and “techoic acids,” (labpaq, p 128. Essay on differential reinforcement 2753 words | 12 pages differential reinforcement is defined to occur when behavior is reinforced by being either rewarded or. Microscopy and differential staining 1what are the advantages and disadvantages of the different types of light and electron microscopes discussed in chapter 3 that are used to study microorganismsmicroscopy and differential staining. Below is an essay on simple and differential staining of bacteria from anti essays, your source for research papers, essays, and term paper examples simple and differential staining of bacteria in previous exercises you have observed live bacteria via compound brightfield microscopy.
Bacterial staining differential staining method – under this type of classification, the staining method employed divides the microorganism into groups we will write a custom essay sample on bacterial staining specifically for you for only $1638 $139/page order. In the lab you use the gram staining procedure, a differential staining technique, as a first step in identifying the type of bacteria on a slide after you carefully perform the staining procedure, you look at the cells under the microscope and see purple rod shaped cells. Grain staining essay 1129 words jul 7th, 2012 5 pages differential staining techniques are used to help identify microorganisms based on the morphology and its ability to accept the stains used during the staining process the cells retaining the initial stain are positive, and the cells retaining the counter or secondary stain are. Any staining procedure that allows differentiation of one type of bacterium from another is a differential staining procedure for all staining procedures, it is first required that cells be fixed to the slide.